abstract：:Two of the most characteristic features of striated muscle are (i) its ability to contract and generate tension when activated and (ii) its ability to return to its original length and form after contraction or stretching ceases. These two properties are to a large extent the primary manifestations of separate sets of...

journal_title：Journal of muscle research and cell motility

authors： Vigoreaux JO

更新日期：1994-06-01 00:00:00

abstract：:We have investigated high-affinity ryanodine-binding sites in membrane preparations from representative fast-twitch and slow-twitch muscles of the rabbit and rat, as well as from human mixed muscle. Our results, obtained in high-ionic strength binding buffer, demonstrate extensive similarities in binding affinity for ...

journal_title：Journal of muscle research and cell motility

authors： Damiani E,Margreth A

更新日期：1994-04-01 00:00:00

abstract：:High speed isovelocity shortening using a servo-controlled lever was performed on isolated whole frog sartorius muscles at long lengths to ensure substantial passive tension. The tension records of unstimulated control experiments were subtracted from the tension records of fully-tetanized experiments on the same musc...

journal_title：Journal of muscle research and cell motility

authors： Seo JS,Krause PC,McMahon TA

更新日期：1994-02-01 00:00:00

abstract：:Invertebrate mini-titins are members of a class of myosin-binding proteins belonging to the immunoglobulin superfamily that may have structural and/or regulatory properties. We have isolated mini-titins from three molluscan sources: the striated and smooth adductor muscles of the scallop, and the smooth catch muscles ...

journal_title：Journal of muscle research and cell motility

authors： Vibert P,Edelstein SM,Castellani L,Elliott BW Jr

更新日期：1993-12-01 00:00:00

abstract：:The characteristics of the titin filament in the vicinity of the Z-line were investigated using immunoelectron microscopy. We used monoclonal titin antibodies T-11 and T-12 on single fibres of frog skeletal muscle, and on Z-line-extracted fibres. It is well established that the I-band region of titin is elastic. We fi...

journal_title：Journal of muscle research and cell motility

authors： Trombitás K,Pollack GH

更新日期：1993-08-01 00:00:00

abstract：:Results were obtained from contracting frog muscles by collecting high quality time-resolved, two-dimensional, X-ray diffraction patterns at the British Synchrotron Radiation Source (SERC, Daresbury, Laboratory). The structural transitions associated with isometric tension generation were recorded under conditions in ...

journal_title：Journal of muscle research and cell motility

authors： Bordas J,Diakun GP,Diaz FG,Harries JE,Lewis RA,Lowy J,Mant GR,Martin-Fernandez ML,Towns-Andrews E

更新日期：1993-06-01 00:00:00

abstract：:In frog semitendinosus muscle the descending limb of the length-tension curve is shifted rightward relative to that of tibialis anterior. Both the plateau right corner and the zero-force intercept are equally shifted. To investigate the reason for this shift, we compared filament lengths in the two muscles. Single fib...

journal_title：Journal of muscle research and cell motility

authors： Trombitás K,Frey L,Pollack GH

更新日期：1993-04-01 00:00:00

abstract：:The sarcomere length (SL) dependence of the calcium sensitivity of force was measured in skinned single twitch and slow-tonic muscle fibres from frog and toad. Twitch and slow-tonic fibres were characterized by location, appearance, physiological response to calcium and by protein band patterns from sodium-dodecyl-sul...

journal_title：Journal of muscle research and cell motility

authors： Martyn DA,Coby R,Huntsman LL,Gordon AM

更新日期：1993-02-01 00:00:00

abstract：:The time course of shortening at zero load was studied by the slack test method during tetanic contractions in isolated, single, slow muscle fibres of the crayfish. In 28 of 32 shortenings (from 14 different fibres) a biphasic shortening was seen, which consisted of an initial high-velocity phase lasting 3.3-20.8 ms a...

journal_title：Journal of muscle research and cell motility

authors： Tameyasu T

更新日期：1992-12-01 00:00:00

abstract：:The effects of 2,3-butanedione monoxime (BDM) on contraction characteristics were studied at 5 degrees C in single intact fibres isolated from the tibialis anterior muscle of the frog. The force-velocity relation was determined using the controlled-velocity method in either whole fibres or short fibre segments in whic...

journal_title：Journal of muscle research and cell motility

authors： Bagni MA,Cecchi G,Colomo F,Garzella P

更新日期：1992-10-01 00:00:00

abstract：:It has been proposed that in striated muscle inositol 1,4,5-trisphosphate (InsP3) may serve as a chemical transmitter linking membrane depolarization to Ca(2+)-release from the sarcoplasmic reticulum. Key to that hypothesis of excitation-concentration (EC) coupling was the observation that skinned muscle fibres contra...

journal_title：Journal of muscle research and cell motility

authors： Hannon JD,Lee NK,Yandong C,Blinks JR

更新日期：1992-08-01 00:00:00

abstract：:Five monoclonal antibodies that react with different regions of myosin light chain 1 from human ventricular myocardial muscle were used to obtain information on interactions between the light chain 1 and heavy chains and generally on the tertiary structure of the light chain 1 within the myosin head. We performed West...

journal_title：Journal of muscle research and cell motility

authors： Cornillon B,Cathiard AM,Eldin P,Anoal M,Cardinaud R,Liautard JP,Le Cunff M,Mornet D,Pons F,Leger J

更新日期：1992-06-01 00:00:00

abstract：:Trabeculae from the right ventricle of rat were chemically 'skinned' with saponin (50 micrograms ml-1 for 30 min). Caffeine (10 mM) induced a transient contracture as a consequence of Ca(2+)-release from the sarcoplasmic reticulum and subsequent activation of the myofilaments. The amplitude of the caffeine contracture...

journal_title：Journal of muscle research and cell motility

authors： Steele DS,Miller DJ

更新日期：1992-04-01 00:00:00

abstract：:We investigated the expression of myosin subunits (myosin heavy chains) as well as light chains and the in vivo phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the adult male European hamster (Cricetus cricetus L.). Two myosin heavy chain isoenzymes could be detected under native a...

journal_title：Journal of muscle research and cell motility

authors： Morano I,Adler K,Agostini B,Hasselbach W

更新日期：1992-02-01 00:00:00

abstract：:The developmental expression of nebulin was studied in embryonic chick skeletal muscle cells in vitro by means of immunofluorescence microscopy. Initially nebulin appeared homogeneously or in a punctate form in the cytoplasm, and then it was assembled into I-Z-I-like complexes containing actin and alpha-actinin but no...

journal_title：Journal of muscle research and cell motility

authors： Komiyama M,Zhou ZH,Maruyama K,Shimada Y

更新日期：1992-02-01 00:00:00

abstract：:When skinned skeletal muscle fibres with rest sarcomere length (L = 2.5 microns) are compressed by the addition of various concentrations ([PVP]) of polyvinylpyrrolidine, the relation between the 1,0 spacing (d) of thick filament lattice and [PVP] has been known to break at d of around 35 nm, resulting in a steeper sl...

journal_title：Journal of muscle research and cell motility

authors： Umazume Y,Higuchi H,Takemori S

更新日期：1991-10-01 00:00:00

abstract：:We have determined the amino acid sequences of the essential light chains (ELC) and regulatory light chains (RLC) of myosin from two species of clam, Mercenaria mercenaria and Macrocallista nimbosa, using protein chemistry methods. The N-termini of all four proteins were blocked, and sequencing was carried out on vari...

journal_title：Journal of muscle research and cell motility

authors： Barouch WW,Breese KE,Davidoff SA,Leszyk J,Szent-Györgyi AG,Theibert JL,Collins JH

更新日期：1991-08-01 00:00:00

abstract：:Myosin heavy chain species were investigated during development in avian pectoralis major muscles (type IIB fibres) by high resolution anion-exchange chromatography of the myosin head region, subfragment-1. At 15 days in ovo four distinct fast-type heavy chain species, I, II, III and IV, in order of elution, were iden...

journal_title：Journal of muscle research and cell motility

authors： Rushbrook JI,Weiss C,Yao TT

更新日期：1991-06-01 00:00:00

abstract：:Although a substantial literature exists on the in vitro polymerization of purified myosin, little is known about native thick filament assembly, remodeling or turnover. We have recently described a cell-free system (Bouche et al., 1988) to examine the interactions between thick filaments and soluble, newly synthesize...

journal_title：Journal of muscle research and cell motility

authors： Goldfine SM,Einheber S,Fischman DA

更新日期：1991-04-01 00:00:00

abstract：:The effect of Ca2+ activation on the apparent rate constant governing the dissociation of force generating myosin crossbridges was studied in skinned rabbit adductor magnus fibres (fast-twitch) at 21 +/- 1 degree C. Simultaneous measurements of Ca2(+)-activated isometric force and ATPase activity were conducted in par...

journal_title：Journal of muscle research and cell motility

authors： Kerrick WG,Potter JD,Hoar PE

更新日期：1991-02-01 00:00:00

abstract：:Creatine kinase (CK) isoenzyme compositions of different types of single muscle fibres dissected from soleus (SOL) and extensor digitorum longus (EDL) muscles from rats were examined. CK isoenzymes were separated into cytoplasmic (CK-MM, CK-MB, CK-BB) (muscle, brain and hybrid types, respectively) and mitochondrial (m...

journal_title：Journal of muscle research and cell motility

authors： Yamashita K,Yoshioka T

更新日期：1991-02-01 00:00:00

abstract：:Cells isolated from pig urinary bladders and pregnant full term human uteruses were attached longitudinally between a microforce transducer and a length displacement apparatus. Cells were stretched by applying a series of ramp-like length changes of 0.2 s duration and 10.0 microns amplitude at intervals of 15 min. Pas...

journal_title：Journal of muscle research and cell motility

authors： Glerum JJ,Van Mastrigt R,Van Koeveringe AJ

更新日期：1990-10-01 00:00:00

abstract：:A transducer has been developed for measuring the minute forces generated during isometric contractions (1.0-10.0 microN) of single smooth muscle cells from the pig urinary bladder and the human uterus. In addition to its high sensitivity, resolution and stability (100 mV microN-1, and less than 0.1 microN and less th...

journal_title：Journal of muscle research and cell motility

authors： Glerum JJ,Van Mastrigt R

更新日期：1990-08-01 00:00:00

abstract：:Monoclonal antibodies (mAb's) have been raised against proteins in preparations of Z-discs isolated from honeybee fibrillar flight muscle. These antibodies have identified four Z-disc antigens on immunoblots of honeybee fibrillar proteins. Antibody alpha binds to the 90-100 kD protein, alpha-actinin; mAb P interacts w...

journal_title：Journal of muscle research and cell motility

authors： Saide JD,Chin-Bow S,Hogan-Sheldon J,Busquets-Turner L

更新日期：1990-04-01 00:00:00

abstract：:A method for quick-freezing muscles while observing their mechanical properties until the moment of freezing is described. This method was used to freeze the anterior byssus retractor muscle (ABRM) of Mytilus edulis. Intact muscle in the presence of sucrose as a cryoprotectant was freeze-substituted in acetone, fixed ...

journal_title：Journal of muscle research and cell motility

authors： Bennett PM,Elliott A

更新日期：1989-08-01 00:00:00

abstract：:The effect of changes in hydrostatic pressure (up to 10 MPa) on the maximally calcium-activated tension in glycerinated rabbit psoas fibres has been examined. The steady active tension was depressed by 0.8% per MPa pressure rise. This pressure sensitivity was enhanced by the pressure of millimolar phosphate and depres...

journal_title：Journal of muscle research and cell motility

authors： Fortune NS,Geeves MA,Ranatunga KW

更新日期：1989-04-01 00:00:00

abstract：:Using a variety of techniques, we have demonstrated the presence of at least two fibre types in Limulus median telson levator muscle. By light and electron microscopy, large (2,156 microns 2 mean cross-sectional area) fibres have A-bands of 4.1 microns, one-half I bands of 2.15 microns and Z lines less than or equal t...

journal_title：Journal of muscle research and cell motility

authors： Levine RJ,Davidheiser S,Kelly AM,Kensler RW,Leferovich J,Davies RE

更新日期：1989-02-01 00:00:00

abstract：:The pattern of spontaneous skeletal muscle degeneration and clinical recovery hindlimb muscles of the mdx mutant mouse was examined for functional and metabolic confirmation of apparent structural regeneration. The contractile properties, histochemical staining and myosin light chain and parvalbumin contents of extens...

journal_title：Journal of muscle research and cell motility

authors： Anderson JE,Bressler BH,Ovalle WK

更新日期：1988-12-01 00:00:00

abstract：:Polyclonal antibodies have been developed against the junctional feet or spanning protein from skeletal muscle triads. These probes in combination with immunogold labels have been used to localize the spanning protein by electron microscope of isolated vesicles from terminal cisternae/triads. The spanning protein anti...

journal_title：Journal of muscle research and cell motility

authors： Kawamoto RM,Brunschwig JP,Caswell AH

更新日期：1988-08-01 00:00:00

abstract：:The properties of extraocular muscles (EOMs) are quite different from those of the trunk and limb. Here we show that there is a novel pattern of troponin T (TnT) expression in EOMs which most likely contributes to the fine control of ocular movement and may reflect their innervation by cranial motoneurons. Three regio...

journal_title：Journal of muscle research and cell motility

authors： Briggs MM,Jacoby J,Davidowitz J,Schachat FH

更新日期：1988-06-01 00:00:00

abstract：:The mechanical events following release of ATP from P3-1-(2-nitro)phenylethyladenosine-5'-triphosphate (caged-ATP) in skinned guinea pig taenia coli smooth muscle in rigor were investigated. A rigor force of about 25-35% of the maximal active force was obtained by removing ATP at the plateau of a maximal active contra...

journal_title：Journal of muscle research and cell motility

authors： Arner A,Goody RS,Rapp G,Rüegg JC

更新日期：1987-10-01 00:00:00

abstract：:Scallop S1 has a region sensitive to tryptic hydrolysis not found thus far in S1s of other species, located 65K from the N-terminus as determined by SDS/polyacrylamide-gel electrophoresis. In the presence of actin the S1 heavy chain is preferentially cleaved at this site. The high-salt EDTA and calcium ATPase activiti...

journal_title：Journal of muscle research and cell motility

authors： Szentkiralyi EM

更新日期：1987-08-01 00:00:00

abstract：:Single twitch muscle fibres have been isolated from various parts of the iliofibularis muscle of Xenopus laevis. After measurements of their isotonic contractile properties, myosin extraction was performed on individual fibres and the extract analysed by various forms of gel electrophoresis. In agreement with previous...

journal_title：Journal of muscle research and cell motility

authors： Lännergren J

更新日期：1987-06-01 00:00:00

abstract：:Myosin heavy chain kinase activity was identified in the high speed supernate of lysed Dictyostelium amoebae and was precipitated by 30-50% ammonium sulphate. In low ionic strength buffer, the activity bound tightly to a Cibacron Blue Sepharose column and eluted as a single peak with 1.0 M NaCl. Gel filtration chromat...

journal_title：Journal of muscle research and cell motility

authors： Kuczmarski ER

更新日期：1986-12-01 00:00:00

abstract：:Purified antibodies to the thick filament accessory proteins, C-protein, X-protein and H-protein, have been used to label fibres of three rabbit muscles, psoas (containing mainly fast white fibres), soleus (containing mainly slow red fibres) and plantaris (a muscle of mixed fibre type) and their location has been exam...

journal_title：Journal of muscle research and cell motility

authors： Bennett P,Craig R,Starr R,Offer G

更新日期：1986-12-01 00:00:00

abstract：:A high resolution, time efficient method is described for determining the complex modulus of activated striated muscle. In this method the applied oscillation of muscle length takes the form of pseudo-random binary noise, PRBN. As a time-domain signal, PRBN is an extension of the double step approach to include n step...

journal_title：Journal of muscle research and cell motility

authors： Rossmanith GH

更新日期：1986-08-01 00:00:00

abstract：:The width (D) and the 1,0 lattice spacing (d1,0) at various ionic compositions of mechanically skinned single fibres (from semitendinosus muscle of Rana catesbeiana) were measured at various concentrations of polyvinyl pyrrolidone (PVP K-30, Mn = 40 000) from 0 to 6% at 20 degrees C. In a standard relaxing solution (4...

journal_title：Journal of muscle research and cell motility

authors： Umazume Y,Onodera S,Higuchi H

更新日期：1986-06-01 00:00:00

abstract：:Heat rate and tension were measured during the steady state of isovelocity shortening in frog sartorius muscles at two speeds (7.5 and 2.0 mm s-1) through two ranges of the length-tension curve: 1.11 to 1.01 L0 and 1.01 to 0.91 L0. Both heat rate and mechanical power were higher at short length than at long length. Th...

journal_title：Journal of muscle research and cell motility

authors： Gilbert SH

更新日期：1986-04-01 00:00:00

abstract：:There is evidence to suggest that 8 nm calcium transport particles in the sarcoplasmic reticulum are involved in the regulation of twitch properties in adult muscles. We have studied ultrastructural characteristics of the sarcoplasmic reticulum in relation to previously defined physiological changes that take place in...

journal_title：Journal of muscle research and cell motility

authors： Gauthier GF,Hobbs AW

更新日期：1986-04-01 00:00:00

abstract：:The efficacy of the 'EGTA-treatment' for producing a model of selectively 'skinned' cardiac muscle has been questioned. This paper deals with ultrastructural evidence designed to test whether small ions can gain access to the myofibrillar space of the heart after 'EGTA-treatment'. Lanthanum has been employed because o...

journal_title：Journal of muscle research and cell motility

authors： Miller DJ,Elder HY,Smith GL

更新日期：1985-10-01 00:00:00